ABSTRACT
Se analizaron diferentes alimentos de origen animal para detectar la presencia de Listeria spp. De 208 muestras de leche cruda de tambo, se obtuvieron 5 capas de Listeria innocua, 1 de L. monocytogenes y 1 de L. welshimeri, tipificadas por pruebas bioquímicas y serológicas. El método de enriquecimiento rápido resultó el de elección y tanto el agar Palcam como el agar Oxford permitieron el crecimeitno de las 7 cepas. L. monocytogenes se recuperó de la leche de un animal con mastitis subclínica. Ninguna de las muestras analizadas de leches pasteurizadas o chocolatada ni de quesos contenía listeria, en cambio en las de helados se recuperó una cepa de L. welshimeri. Para los alimentos cárnicos, el empleo de enriquecimiento en 2 etapas facilitó la detección de Listeria spp. Se observó un predominio de L. ivanovii en el 2,5 por ciento de las muestras
Subject(s)
Food Contamination/analysis , Listeria/isolation & purification , Meat Products/microbiology , Dairy Products/microbiology , ArgentinaABSTRACT
Recent food listeriosis outbreaks confirm that more faithful isolation and identification methods for Listeria monocytogenes or other potentially pathogen microorganisms are required. Furthermore, the human and animal reservoir role in the ecology of this disease must be established. Listeria spp. in the vizcacha intestinal content was determined by two isolation procedures, starting from 10 g of homogenized samples in 40 ml of PBS. I)0.1 ml was stripped on phenylethanol agar, selective agar for Listeria and acryflavin ceftazidin agar, then incubated at 37 degrees C for 48 h, suspected colonies were identified by preliminary tests (Gram, hemolysis, catalase, esculin hydrolisis and motility at 22 degrees C) and confirmatory tests (indol, methyl red, Voges Proskauer, nitrate and carbohydrate fermentation) (Table 1). Antibiotic susceptibility, protein profile by PAGE and pathogenic power in mice were determined. II) The remaining homogenate was incubated at 4 degrees C in 100 ml of Donnelly and Baigent enrichment broth, weekly or monthly with subcultures until 30 days or 6-8 months, respectively. The subcultures were followed up as in I). A L. seeligeri strain, susceptible to antibiotics suggested for L. monocytogenes and exhibiting resistance to some second and third generation cephalosporins, was isolated (Table 2). The protein profile of both species was coincident, but L. seeligeri was not virulent for mice. The finding of L. seeligeri in an animal (4.0
) used as human feeding source is of interest due to its potential pathogen power.
ABSTRACT
In order to detect subclinical mastitis by means of California Mastitis Test and recounting of somatic cells, 163 cows from the dairies of San Luis city, Argentina, were examined. Seventy six individuals (46.6
) exhibited an inflammatory response ranging > or = 2+ grade and a cellular recounting value of > or = 5 x 10(5), data compatible with those of subclinical mastitis. Staphylococcus aureus was isolated from 39 (51.3
) cultures as estimated by the sum of the two last values listed in Table 1. Organisms were isolated by plating on brain heart infusion agar with 5
of sheep blood and on Baird-Parker media. One hundred and three S. aureus isolates recovered from 51 of 63 cows were characterized by coagulase activity by the tube method using human and bovine plasma; clumping factor; glucose and mannitol fermentation; thermonuclease (TNase), pigment, gelatinase, fibrinolysin, acetoin, hemolysin production; egg yolk, tellurite and catalase reaction and crystal violet types. All isolates were susceptible to cephalothin, clindamycin, methicillin, gentamycin and vancomycin; 94.1
were susceptible to chloramphenicol and 53.8
) were classified according to Hájek and Marsálek scheme as biotype C (bovine and ovine ecovar), 33 isolates (32.0
) were classified as biotype B (swine and poultry ecovar); 1 isolated (0.9
) as intermediate between B and D; 5 isolates (4.8
) as biotype A (human ecovar) and 1 isolated (0.9
) as biotype D (ecovar silvestres spp) (Table 2). Production of enterotoxins A to E and toxic shock syndrome toxin-1 (TSST-1) was determined by the optimal susceptibility plate method on 27 isolates (26.2
) which were coagulase 3+ to 4+ and TNase highly positive. None of them produced enterotoxins including TSST-1. The subclinical mastitis data and the prevalence of S. aureus coincide with those of other authors, both from Argentina and from other countries.
ABSTRACT
In order to detect subclinical mastitis by means of California Mastitis Test and recounting of somatic cells, 163 cows from the dairies of San Luis city, Argentina, were examined. Seventy six individuals (46.6
) exhibited an inflammatory response ranging > or = 2+ grade and a cellular recounting value of > or = 5 x 10(5), data compatible with those of subclinical mastitis. Staphylococcus aureus was isolated from 39 (51.3
) cultures as estimated by the sum of the two last values listed in Table 1. Organisms were isolated by plating on brain heart infusion agar with 5
of sheep blood and on Baird-Parker media. One hundred and three S. aureus isolates recovered from 51 of 63 cows were characterized by coagulase activity by the tube method using human and bovine plasma; clumping factor; glucose and mannitol fermentation; thermonuclease (TNase), pigment, gelatinase, fibrinolysin, acetoin, hemolysin production; egg yolk, tellurite and catalase reaction and crystal violet types. All isolates were susceptible to cephalothin, clindamycin, methicillin, gentamycin and vancomycin; 94.1
were susceptible to chloramphenicol and 53.8
) were classified according to Hájek and Marsálek scheme as biotype C (bovine and ovine ecovar), 33 isolates (32.0
) were classified as biotype B (swine and poultry ecovar); 1 isolated (0.9
) as intermediate between B and D; 5 isolates (4.8
) as biotype A (human ecovar) and 1 isolated (0.9
) as biotype D (ecovar silvestres spp) (Table 2). Production of enterotoxins A to E and toxic shock syndrome toxin-1 (TSST-1) was determined by the optimal susceptibility plate method on 27 isolates (26.2
) which were coagulase 3+ to 4+ and TNase highly positive. None of them produced enterotoxins including TSST-1. The subclinical mastitis data and the prevalence of S. aureus coincide with those of other authors, both from Argentina and from other countries.